Figure 4

Suppression of the reg6 embryonic phenotype by SKF91488 and histamine. (A, bright field; B, GFP) Untreated reg6 embryos developed dilated caudal veins at 33°C (yellow arrows). (C, bright field; D, GFP) reg6 embryos treated with 10 μM HMT inhibitor SKF91488 developed normal caudal veins. Anterior, left; dorsal, top. (E) Typically, almost 70% of reg6 embryos formed dilated caudal veins at 33°C (blue bar). The penetrance was reduced by SKF91488 (red bars, n = 50) and histamine (green bars, n = 50).