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Figure 4 | BMC Developmental Biology

Figure 4

From: Teneurin-1 is expressed in interconnected regions of the developing brain and is processed in vivo

Figure 4

Teneurin-1 appears to be processed in the developing chicken brain. Western blot analysis of embryonic chicken brain lysates. Detection of endogenous teneurin-1 using anti-ICD (A) and anti-ECD antibodies (B). The numbers to the left indicate the positions of molecular weight standards. The anti-ICD detects a prominent N-terminal fragment at 65 kDa (arrowhead), which is much smaller than expected for the full-length protein (arrow), suggesting proteolytic processing. In contrast, using the anti-ECD antibody results in detection of the full-length protein (arrow) and a large C-terminal fragment (arrowhead). Asterisks label additional processing products (see text for details). Postulated cleavage sites and calculated molecular weights of processing products (C). Postulated cleavage sites: 1 = ectodomain shedding, 2 = intramembrane cleavage releasing the ICD. The sizes indicated are predicted from the amino acid sequence; known apparent molecular weights are shown in parentheses (see text for details). The calculated molecular weights are approximate, as the exact cleavage sites are not known.

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