Figure 1

Teneurin-1 is expressed in many parts of the developing CNS. In situ hybridization at E17 with a teneurin-1 antisense probe (sense controls were negative). In the olfactory bulb (A), there is a strong hybridization signal in the mitral cell layer (mcl). The ganglion cell layer (gcl) is also positive, but the external plexiform layer (epl) is not. In the retina (B), the ganglion cell layer (gcl) is labelled intensely, and there is a faint signal in neurons of the inner nuclear layer (inl) adjacent to the inner plexiform layer (ipl). The pigment retina (pr) has dark melanosomes. The nucleus rotundus (C) contains large, scattered neurons that are positive for teneurin-1 mRNA. In the optic tectum (D) teneurin-1 mRNA is widespread, but is seen most clearly in the large neurons of the stratum griseum centrale (sgc). In the hindbrain (E) the nucleus laminaris (La) and nucleus magnocellularis (MCC) are labelled, as are Purkinje cells (Pcl) and other neurons in the cerebellum (F).