Table 1 Quantitative assessment of phenotypic rescue of moe- mutant defects by injection of moe and epb4.1l5 mRNA constructs.
Injected mRNA Construct | Genetic Background | % Edema | % Brain Ventricle Defects | % RPE Defects | Apical ZO-1/panCrb | Retinal Lamination |
|---|---|---|---|---|---|---|
- | wt control | 0% (0/81) | 0% (0/81) | 0% (0/81) | Yes | Yes |
- | moe+/- incross | 25% (22/88) | 25% (22/88) | 25% (22/88) | No | No |
moe | moe+/- incross | 24% (8/34) | 0% (0/34) | 0% (0/34) | Yes | Yes |
epb4.1l5 long | moe+/- incross | 25% (6/24) | 0% (0/24) | 0% (0/24) | Yes | Yes |
epb4.1l5 short | moe+/- incross | 27% (10/37) | 27% (10/37) | 24% (9/37) | No | No |
epb4.1l5 FERM | moe+/- incross | 24% (4/17) | 24% (4/17) | 24% (4/17) | No | No |
epb4.1l5 long_ΔPBD | moe+/- incross | 26% (30/86) | 26% (30/86) | 26% (30/86) | No | No |
epb4.1l5 short+long_PBD | moe+/- incross | 19% (9/48) | 23% (11/48) | 0% (0/48) | Yes | Yes |
epb4.1l5 long+short_PBD | moe+/- incross | 61% (20/47) | 96% (54/56) | 1% (1/88)* | Yes | Yes |
epb4.1l5 long | wt | 0% (0/16) | 0% (0/16) | 0% (0/16) | NA | NA |
epb4.1l5 long+short_PBD | wt | 20% (7/35) | 42% (32/63) | 3% (1/35) | NA | NA |