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Table 1 Quantitative assessment of phenotypic rescue of moe- mutant defects by injection of moe and epb4.1l5 mRNA constructs.

From: Tissue-specific requirements for specific domains in the FERM protein Moe/Epb4.1l5 during early zebrafish development

Injected mRNA Construct Genetic Background % Edema % Brain Ventricle Defects % RPE Defects Apical ZO-1/panCrb Retinal Lamination
- wt control 0% (0/81) 0% (0/81) 0% (0/81) Yes Yes
- moe+/- incross 25% (22/88) 25% (22/88) 25% (22/88) No No
moe moe+/- incross 24% (8/34) 0% (0/34) 0% (0/34) Yes Yes
epb4.1l5 long moe+/- incross 25% (6/24) 0% (0/24) 0% (0/24) Yes Yes
epb4.1l5 short moe+/- incross 27% (10/37) 27% (10/37) 24% (9/37) No No
epb4.1l5 FERM moe+/- incross 24% (4/17) 24% (4/17) 24% (4/17) No No
epb4.1l5 long_ΔPBD moe+/- incross 26% (30/86) 26% (30/86) 26% (30/86) No No
epb4.1l5 short+long_PBD moe+/- incross 19% (9/48) 23% (11/48) 0% (0/48) Yes Yes
epb4.1l5 long+short_PBD moe+/- incross 61% (20/47) 96% (54/56) 1% (1/88)* Yes Yes
epb4.1l5 long wt 0% (0/16) 0% (0/16) 0% (0/16) NA NA
epb4.1l5 long+short_PBD wt 20% (7/35) 42% (32/63) 3% (1/35) NA NA
  1. Wild-type embryos or embryos from heterozygous moe+/- incrosses were injected with the indicated mRNA constructs and scored based on pericardial edema, brain ventricle defects, RPE defects, apical localization of ZO-1 or Crb2a at 30 hpf, and retinal lamination at 5 dpf. Phenotypes that were not analyzed are indicated with an NA. * See Figure 4M, N and text.