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Figure 2 | BMC Developmental Biology

Figure 2

From: Function of the PHA-4/FOXA transcription factor during C. eleganspost-embryonic development

Figure 2

PHA-4 is required for dauer recovery, gonad and vulva development. (A) pha-4 RNAi treatment efficiently inhibits pha-4 transcription. Semi-quantitative RT-PCR shows pha-4 and rpl-21 transcription levels in daf-7(e1372) and daf-2(e1370) dauer larvae treated with the RNAi control (lanes 1, 3) and pha-4 RNAi (lanes 2, 4). RT-PCR experiments were performed three times with consistent results using three independent RNA preparations. (B) PHA-4 is needed for dauer recovery. Dauer larvae formed constitutively at 25°C in the presence of pha-4 RNAi from hatching showed significantly decreased recovery upon downshift to 15°C compared to control daf-7(e1372), daf-1(m40), daf-2(e1370), and age-1(m865) mutants. Percentages of dauer recovery, numbers of animals scored, and p values for t-tests were shown in Table 1. The entire experiment was performed twice with triplicates for each treatment. (C-F) pha-4 is involved in gonad and vulva development. (C) Control post-dauer daf-7(e1372) adult; (D) pha-4 RNAi-treated post-dauer daf-7(e1372) adult; (E) control post-dauer daf-2(e1370) adult; and (F) pha-4 RNAi-treated post-dauer daf-2(e1370) adult. The Daf-c mutants all exhibit similar gonad and vulva defects with variable expressivity. Anterior is left and dorsal is up. dtc, distal tip cell; germ, germline; oo, oocytes; emb, embryos; vul, vulva. Scale bars, 10 μm.

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