Figure 1

pha-4 transcripts are enriched in dauer larvae. Semi-quantitative RT-PCR experiments were performed to determine levels of pha-4 and rpl-21 mRNA. rpl-21, which encodes a ribosomal protein, serves as the internal control for equal loading. Experiments were performed three times with consistent results using three independent RNA preparations. Lane 1, mixed stages; lane 2, dauer; lane 3, 4 and 5, 2 hours, 24 hours and 36 hours after placement in food, respectively. Dauer larvae begin to feed approximately three hours after exposure to food.