Figure 6
From: PITX2 gain-of-function induced defects in mouse forelimb development
Unchanged expression of Ccnd2 and no differences in apoptotic activity in the Ktcn-PITX2 forelimbs. (A and B) H&E stained sagittal paraffin sections of forelimbs from E14.5 mice. The marked areas in A and B are magnified in C-H. (C-F) Double labeling immunohistochemistry reveals that the cells over-expressing PITX2 (D) do not display an increased expression of Ccnd2 (F). (G and H) TUNEL staining of sections from wild type and Ktcn-PITX2 forelimbs does not show any difference in apoptotic activity in the cells over-expressing PITX2. Scale bar: 0.5 mm.