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Figure 6 | BMC Developmental Biology

Figure 6

From: Expression of the Ladybird-like homeobox 2 transcription factor in the developing mouse testis and epididymis

Figure 6

Lbx2 expression in postnatal testis. In situ hybridization (ISH) experiments using DIG-labeled antisense (A, C, D, E, F) and sense (H) Lbx2 cRNA probes were performed on 6 μm paraffin sections of paraformaldehyde-fixed mouse testis. Lbx2 mRNA was detected by immunostaining using an alkaline phosphatase-coupled anti-DIG antibody (appears as a blue-purplish staining). Tissues were counterstained with Neutral Red to visualize nuclei. Lbx2 expression in the post-natal testis was assessed at P5 (A), P32 (C), P34 (D), P36 (E), and P70 (F). By immunohistochemistry (IHC), the Sertoli cell cytoplasm in P5 (B) and P70 (G) testis was labeled using an anti-MIS antiserum and revealed using AEC (shows as a red-brownish staining). Expression of Lbx2 is evident within the cytoplasm of Sertoli cells at all ages. In interstitial cells, a weak but consistent staining is observed at P5 (A) while a strong signal is detected at P32, P34, and P36 (C-E). At P70, some germ cells are also labeled for Lbx2 (F). No significant signal was detected with a sense probe as shown in the adult (H) section. Gc: germ cells, L: Leydig cells, S: Sertoli cells. Magnifications: 200× (A, B), 400× (C-H).

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