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Figure 4 | BMC Developmental Biology

Figure 4

From: Expression of the Ladybird-like homeobox 2 transcription factor in the developing mouse testis and epididymis

Figure 4

Lbx2 is strongly expressed in mesonephric cells and in seminiferous tubules of the developing testis. Six μm paraffin sections of paraformaldehyde-fixed mouse developing urogenital ridge (E14) were probed with DIG-labeled antisense (left panel) and sense (right panel) Lbx2 cRNA probes in in situ hybridization experiments. Lbx2 mRNA was detected by immunostaining using an alkaline phosphatase-coupled anti-DIG antibody (appears as a blue-purplish staining). Tissues were counterstained with Neutral Red to visualize nuclei. Lbx2 mRNA was detected in cells of the Wolffian duct (WD), of the degenerating Müllerian duct (MD), and in the developing seminiferous tubules (ST, outlined by dotted lines). Magnification: 200×.

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