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Figure 8 | BMC Developmental Biology

Figure 8

From: FGF10 maintains distal lung bud epithelium and excessive signaling leads to progenitor state arrest, distalization, and goblet cell metaplasia

Figure 8

Beta-catenin activation and upregulation of BMP4 expression. A-B: IHC using an antibody that is specific for activated beta-catenin dephosphorylated on Ser37 or Thr4, and an antibody that recognizes only membrane bound beta-catenin. Counterstained with Hoechst (hst). A: Wildtype alveolar lung contains scattered cells with activated beta-catenin. B: Beta-catenin is activated in more cells in the transgenic lung. A': magnification from A. B' Magnification from B. In both WT and TG, cells are double positive for activated and membrane-bound beta-catenin, implying that cells retain membrane-bound beta-catenin even when activated. C-D: ISH using a probe for Bmp4. Bmp4 was expressed in both wildtype (C) and transgenic (D) distal lung epithelium, but transgenic expression was much more widespread. Part of the right atrium (Bmp4 negative) is seen to the right hand side of the TG lung lobe in D. E: Multiplex-RTPCR of E14.5 wildtype and transgenic lung using primers for G6pd-2 (Glucose-6-phosphate dehydrogenase gene), Bmp4 and Bmp5 shows upregulation of Bmp4 mRNA.

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