Figure 7

Goblet cell metaplasia in the alveolar epithelium. A-B: IHC with Rhodamine-conjugated Dolichos Biflorus Agglutinin (DBA) and Beta-catenin. Goblet cells detected by their DBA reactivity were present in the alveolar epithelium of the transgenic mice (B), while no goblet cells were detected anywhere in the wildtype lungs (A). C-D: ISH using a Trefoil factor 3 probe. No Tff3 was detected in wildtype lung. Goblet cells in the transgenic lung were positive (D). E-F: IHC for pro-Surfactant protein C and fluorescein conjugated Dolichos Biflorus Agglutinin (DBA). Goblet cells did not co-express pro-Surfactant protein C.