Figure 6
Attenuation of mature lung bronchial cells. IHC on E18.5 embryos. A-C: Uteroglobin, PCNA and smooth muscle actin IHC. Showing distal part of transgenic lung which has one Fgf10FLAG negative untransformed lobe ("WT" lobe), and one lobe expressing Fgf10FLAG (TG). No uteroglobin positive Clara cells are seen in the distal TG lung (A and C), while bronchioles in the "WT" lobe contain Clara cells (A and B). D-E: Uteroglobin, E-cadherin and smooth muscle actin IHC oriented with most proximal end in upper right corner. D: WT proximal lung. E: TG proximal lung. Arrow in inset points to Uteroglobin negative cells. F-G: SOX2 and E-cadherin IHC on distal lung. F: WT bronchiole with SOX2 positive ciliated cells. G: TG lung with a few scattered Sox2 positive cells. H-I: CGRP and E-cadherin IHC. H: WT bronchus with clusters of PNECs. I: TG lung with scattered CGRP positive cells.