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Figure 5 | BMC Developmental Biology

Figure 5

From: FGF10 maintains distal lung bud epithelium and excessive signaling leads to progenitor state arrest, distalization, and goblet cell metaplasia

Figure 5

Alveolar differentiation is attenuated and early distal bud markers upregulated. A-B: TG lung with non FGF10FLAG expressing lobe ("WT") and transgene expressing lobe (TG). A: Pro-surfactant protein C IHC. B: Pro-surfactant protein C and RAGE IHC. C-D: High magnification from A. E-F: High magnification from B. G-H: PCNA and pro-surfactant protein C IHC. G: WT lung, with no PCNA positive alveolar type II cells. H: TG lung with double positive cells. I-J Pea3 and pro-surfactant protein C IHC. I: WT lung with no Pea3 positive alveolar type II cells. J: TG lung with several double positive cells. K-L: TTF1 IHC on WT (K) and TG (L) show expression mainly in TG cells. M-N: Clusterin IHC on WT (M) and TG (N) show expression only in TG cells facing the lumen. O-P: pro-surfactant protein C IHC on WT (O) and TG (P). Q: merge of K, M and O. R: Merge of L, N and P shows triple positive cells. S-V: E16.5 WT lung S: TTF1 IHC. T: Clusterin IHC. U: Pro-surfactant protein C IHC. V: merge of S-U demonstrates that TTF1, clusterin and p-SftpC are co-expressed in the distal bud (arrowhead in S-V and inset in V) at E16.5.

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