Figure 1
Expression of pPdx1- Fgf10FLAG. A: Comparison of normal lung, left, and transgenic, right at E18.5. The transgenic lungs are larger, and exhibit an enlarged branching pattern, with increased lobular distance. B: higher magnification of normal lung, showing the regularly spaced terminal branches, with only minor grooves between lobules. C: transgenic lung at similar magnification, showing enlarged grooves between terminal branches, where individual lobules are covered with a significantly increased mesenchymal tissue layer. D-J: Lung Expression of pPdx1-Fgf10FLAG. D: FGF10 staining on E18.5 TG embryo showing the lobe specific expression of the transgene in this embryo. E: FLAG staining on E18.5 WT and F: TG. Arrow in F indicates a cell with high cytoplasmic FLAG staining while the arrowhead indicates a cell with only membrane staining. G: 26 cycle MPX RT-PCR of E14.5 and E18.5 lung (unable to detect Fgf10 in lung epithelium of E12.5) using primers for Fgf10 and G6pd-2 (Glucose-6-phosphate dehydrogenase gene). H-J: Fgf10 ISH on TG lung and WT littermate, arrows point to Fgf10 positive epithelial cells. H-I: E18.5 WT (H) and TG (I). J: E14.5 TG.