Parthenogenetic activation of bovine oocytes using bovine and murine phospholipase C zeta

Table 3 Optimization of mouse PLCZ1 cRNA concentration to activate bovine oocytes.

Experiment	mPLCZ1 cRNA concentration	Replicates	Oocytes injected	Cleavage Rate¹	Blastocyst Rate¹
Tenth dilutions	0.5 μg/μL	3	123	90.2^a	32.5^a
	0.05 μg/μL	3	112	83.9^b	8.9^b
	0.005 μg/μL	3	122	48.4^c	0.8^c
Refined dilutions	1 μg/μL	3	107	79.4^a	33.6^a
	0.5 μg/μL	3	112	92.0^a	33.9^a
	0.25 μg/μL	3	110	85.5^a	33.6^a

^a,b,c: Percentages not sharing a common letter within experiment are statistically different (P < 0.05, Chi-square test).
¹ Percentage from activated oocytes

ISSN: 1471-213X