Parthenogenetic activation of bovine oocytes using bovine and murine phospholipase C zeta

Table 2 Optimization of bovine PLCZ1 cRNA concentration to activate bovine oocytes.

Experiment	bPLCZ1 cRNA concentration	Replicates	Oocytes injected	Cleavage Rate¹	Blastocyst Rate¹
Tenth dilutions	1 μg/μL	3	110	49.1^a	12.7^a
	0.1 μg/μL	3	118	87.3^b	29.7^b
	0.01 μg/μL	3	112	69.6^a	15.2^a
Refined dilutions	0.5 μg/μL	4	144	70.8^a	18.8^a
	0.1 μg/μL	4	154	88.3^ab	29.9^a
	0.05 μg/μL	4	145	89.0^b	22.8^a

^a,b: Percentages not sharing a common letter within experiment are statistically different (P < 0.05, Chi-square test).
¹ Percentage from activated oocytes.

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