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Figure 2 | BMC Developmental Biology

Figure 2

From: Genetic analysis of the two zebrafish patched homologues identifies novel roles for the hedgehog signaling pathway

Figure 2

Phenotypic consequences of zebrafish ptc1 mutants. Homozygous ptc1hu1602mutants show a subtle somite phenotype at 32 hpf, where the average angle of the somite becomes more obtuse (A,B). At 72 hpf, ptc1hu1602mutants exhibit an eye phenotype where the pigmented epithelium is extended into the diencephalon. The similar phenotype described for the blw mutant is weaker compared to the ptc1hu1602mutant (C-E). The expression level of ptc1, a general readout for Hh activity, shows a mild increase in the blw mutant compared to wild type (F-H). The ptc1hu1602mutant shows a severely increased level of ptc1, where wild types, heterozygotes and mutants can be distinguished based on ptc1 levels (I-K). Additional to the difference in the strength of the eye phenotype, the activation of the pathway is significantly higher in ptc1hu1602mutants compared to blw mutants. An increased expression level of gli1 confirms an activation of the Hh pathway in the ptc1hu1602mutant (L-N).

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