Aberrant epigenetic changes and gene expression in cloned cattle dying around birth

Table 6 Expression of four genes in five organs of dead cloned cattle compared to normal control^a

Gene	Organ Group	Heart	Liver	Spleen	Lung	Kidney
β-actin	N	1.00 ± 0.37	1.00 ± 0.50	1.00 ± 0.60	1.00 ± 1.08	1.00 ± 0.72
	ED	4.44 ± 0.40^b	0.89 ± 0.32	0.20 ± 0.03^b	0.21 ± 0.01	0.41 ± 0.10
	LD	2.57 ± 1.61	0.45 ± 0.36	0.45 ± 0.27	2.05 ± 2.57	0.34 ± 0.15
VEGF	N	1.00 ± 0.42	1.00 ± 0.91	1.00 ± 0.44	1.00 ± 0.39	1.00 ± 0.79
	ED	3.62 ± 2.33	2.92 ± 1.33	1.89 ± 0.04^b	0.96 ± 0.20	4.55 ± 1.37^b
	LD	1.90 ± 1.14	1.13 ± 0.32	0.49 ± 0.21	0.63 ± 0.25	2.35 ± 0.69
H19	N	1.00 ± 0.48	1.00 ± 0.35	1.00 ± 0.31	1.00 ± 1.13	1.00 ± 0.72
	ED	10.00 ± 3.67^c	26.38 ± 30.17	1.51 ± 1.08	0.54 ± 0.26	1.83 ± 1.51
	LD	0.71 ± 0.16	0.08 ± 0.04	0.17 ± 0.14	0.53 ± 0.71	1.91 ± 0.91
Igf2	N	1.00 ± 0.25	1.00 ± 0.09	1.00 ± 0.60	1.00 ± 0.51	1.00 ± 0.93
	ED	18.97 ± 12.83^b	9.85 ± 11.83	1.49 ± 0.42	1.02 ± 0.28	2.18 ± 2.54
	LD	1.15 ± 0.21	1.05 ± 0.77	0.40 ± 0.59	0.21 ± 0.12^b	0.91 ± 0.37

^a The relative trancripts in two groups compared with normal control (the relative trancripts of normal control were normalized to 1). Values are presented as the mean ± SEM. N denotes normal control, ED denotes early death group and LD denotes late death group.
^b (p < 0.05), ^c (p < 0.01) denote significant differences between ED and N, LD and N.

ISSN: 1471-213X