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Table 1 Antigen, species, immunogen, working dilution, and source of the antibodies used in the current study.

From: Muscarinic signaling influences the patterning and phenotype of cholinergic amacrine cells in the developing chick retina

Antigen

Species

Clone/catalogue#/Supplier

Working dilution

AP2α

mouse

3B5/Developmental Studies Hybridoma Bank

1:50

Bromdeoxyuridine (BrdU)

rat

BUI/75/Accurate Chemicals and Scientific Corporation

1:400

Brn3a

mouse

MAB1585/Chemicon

1:1500

Choline acetyltransferase (ChAT)

rabbit

1456/Dr. Miles Epstein, University of Wisconsin

1:1500

Calretinin

Rabbit

7699/4/SWANT

1:1000

Cellular Retinoic Acid Binding Protein (CRABP) (CRABP)

mouse

C1/Dr. Jack Saari, University of Washington

1:1000

Islet1

mouse

40.2D6/Developmental Studies Hybridoma Bank

1:50

Neurofilament (NF) (160 kDa)

mouse

RMO270/Dr. V. Lee, University of Pennsylvania

1:2000

p27kip1

mouse

Clone 57/Transduction Laboratories

1:200

Pax6

mouse

PAX6/Developmental Studies Hybridoma Bank

1:50

Prox1

rabbit

Dr. Stanislov Tomarev, NIH

1:800