Figure 2From: Muscarinic signaling influences the patterning and phenotype of cholinergic amacrine cells in the developing chick retinaCholinergic amacrine cells express low levels of Pax6 and high levels of p27kip1 compared to other amacrine cells. Vertical sections of chick retina were labeled with antibodies to Pax6 (green; a, c, d and f) or p27kip1 (green; g, i, j and l) and ChAT (red). The tissues were obtained from chicks at E7 (a-c and g-i) and E11 (d-f and j-l). Arrows indicate cholinergic amacrine cells that are labeled for Pax6 or p27kip1, and small double arrows indicate p27kip1-negative cells in the progenitor cell layer of the E7 retina. The calibration bar (50 μm) in panel l applies to all panels. Abbreviations: ChAT – choline acetyltransferase; INL – inner nuclear layer; IPL – inner plexiform layer; GCL – ganglion cell layer.Back to article page