Table 1 Genotype of colonies isolated after recombineering
Gene | Cassette | PCR product electroporated | Colonies Obtained | GFP + colonies | Cassette + colonies |
|---|---|---|---|---|---|
K10C2.4 | galk | FLAG-GFP | 714 | 40/48 | 3/48 |
K10C2.4 | galk | None | 85 | 0/48 | 23/48 |
K10C2.4 | RT | FLAG-GFP | 55 | 0/48 | 46/48 |
K10C2.4 | RT | None | 81 | 0/48 | 46/48 |
dod-22 | galk | FLAG-GFP | N.D. | 46/48 | N.D. |
dod-22 | RT | FLAG-GFP | N.D. | 1/48 | N.D. |
myo-3 | galk | FLAG-GFP | N.D. | 46/47 | N.D. |
myo-3 | RT | FLAG-GFP | N.D. | 2/48 | N.D. |
dod-22 (Frozen 1 year) | galk | FLAG-GFP | N.D. | 16/48 | N.D. |
myo-3 (Frozen 1 year) | galk | FLAG-GFP | N.D. | 18/48 | N.D. |