Figure 5
Comparison of fosmid modification using pMOD4- galK -G with pMOD4-RT-G. Fosmids carrying the dod-22 (top) or myo-3 genes (bottom) were modified to generate FLAG-GFP fusion genes through positive and negative selection using either the galK or RT cassette. The effectiveness of fosmid modification was monitored via PCR using the indicated oligo sets. Lane 1, 2, 3, 4, 7 and 8 FLAG-GFP F and FLAG-GFP R; Lane 5 and 6 RT screen F and RT screen R.