Figure 1

Generation of Eya3 -deficient mice by insertional mutagenesis. (a) Schematic illustration of the gene trap vector integration into intron 7 of the Eya3 gene resulting in a loss of the following exons. (b) Sequence fragment of the Eya3 gene. The upper chromatogram displays the sequence of wild-type mice, while the lower chromatogram shows the vector integration in mutant mice at 132.23 Mb on chromosome 4 (Ensembl database, release No. 50, 2008). (c) Eya3 mutant mice were genotyped by triplex PCR with a genomic forward primer combined with a genomic reverse primer for identification of wild-type animals and with a reverse primer within the gene trap vector for identification of homozygous mutant animals.