Figure 6

Early or prior mating increases female fecundity. Females were mated once at 3 days post-eclosion (Once₃) or at 10 days post-eclosion (Once₁₀) or twice at 3 and 10 days post-eclosion (Twice_3&10). Eggs were counted at four time points: 6 hr, 1, 2 and 3 days post-mating. (A) Represents the average number of eggs laid by the female during the three days examined. Fertility was calculated as the average number of adult eclosed per laid eggs per female per day. (B) Represents the percentage of female fertility during the three days examined. We examined three independent biological replicates for each mating regime; n = 40–163 females for each replicate. See also additional file 5.