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Figure 5 | BMC Developmental Biology

Figure 5

From: Tissue remodeling: a mating-induced differentiation program for the Drosophilaoviduct

Figure 5

Mating induces changes in the level of cytoskeletal proteins in the oviduct. (A) The abundance of cytoskeletal proteins in oviducts dissected at different times post-mating and following different mating regimes was analyzed by Western blot. We calculated the abundance of each protein in mated females (described below) relative to the level in 3-day-old unmated females. Three different mating regimes were examined: (i) 3-day-old females that mated once at 3 days of age (once3), (ii) 10-day-old females that mated once at 3 days of age (Once3 day 10), and (iii) females that mated twice, first at 3 days and again at 10 days of age (Twice3&10). (B) We also calculated the abundance of each protein in the oviducts of: (i) 5-day-old unmated females relative to its level in oviducts of UM3 (UM5); (ii) 10-day-old unmated females relative to its level in oviducts of UM3 (UM10). For each treatment oviducts were obtained from 3–4 biological replicate; in each replicate for each treatment n = 60 oviducts/lane.

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