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Figure 9 | BMC Developmental Biology

Figure 9

From: The C. elegansEMAP-like protein, ELP-1 is required for touch sensation and associates with microtubules and adhesion complexes

Figure 9

ELP-1 antigens co-pellet with paclitaxel-stabilized C. elegans microtubules. Paclitaxel-stabilized microtubules (Mt) were prepared from a mixed-stage worm extract (Ex) as described in the Materials and Methods. The panel on the left shows the protein complexity in a C. elegans extract (Ex, 25,000 g supernatant) and in a Paclitaxel-stabilized microtubule preparation (Mt, 25,000 g pellet). An ~100 kDa polypeptide and α and β tubulin are the most prominent proteins visualized in the microtubule preparation. Proteins were separated on an 8% acrylamide mini-gel (left panel) and probed with affinity-purified anti-ELP-1 antibodies on a nitrocellulose blot (right panel). Asterisk is next to the ~100 kDa band that cross-reacts with the anti-ELP-1 antibodies. Molecular masses × 10-3 (Mr) are shown to the left. Approximately 15 μg of protein was loaded in each lane of a 7% polyacrylamide SDS-page mini-gel.

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