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Figure 5 | BMC Developmental Biology

Figure 5

From: The Lc3-synthase gene B3gnt5is essential to pre-implantation development of the murine embryo

Figure 5

Glycolipid analysis in ES cells. A. The oligosaccharide moiety of ES cell GSL was cleaved by ceramide glycanase, labeled with 2-aminobenzamide and separated by normal phase HPLC. The retention times of glucose units (GU) is indicated on the x-axis. The dotted line shows the GSL profile of wildtype R1 ES cells and the plain line shows the GSL profile of the R1 clone 5B5. B. Mass spectrometric fragmentation spectrum of the Lc6 oligosaccharide obtained from the corresponding HPLC fraction. The main fragment ions are labeled and their assignment to the Lc6 structure is depicted.

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