Figure 3
From: The Lc3-synthase gene B3gnt5is essential to pre-implantation development of the murine embryo
N-acetylglucosaminyltransferase activity in ES cells. ES cells of the R1 and TC1 lines were lysed and N-acetylglucosaminyltransferase (GlcNAcT) activity was measured towards the acceptor substrates Gal(β-0)p-nitrophenol (pNP) and lactosylceramide (Lac-Cer). Black bars show GlcNAcT activity of wildtype ES cells and open bars show GlcNAcT activity of B3gnt5-targeted ES cells. Each value represents the average + S.D. of three independent measurements.