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Table 2 GFP expression in transient sox10:GFP zebrafish containing named zebrafish sox10 promoter deletions

From: An evolutionarily conserved intronic region controls the spatiotemporal expression of the transcription factor Sox10

 

A

B

 

1)

2)

3)

4)

5)

1)

2)

3)

4)

Pigment cells

84.2

80

82.9

15.4

0

90.1

45

66.7

76.2

Ear

81.6

74.2

53.7

28.2

0

70

90

54.2

66.7

Nasal

78.9

91.2

90.2

51.2

0

36.3

15

8.3

33.3

Facial cartilage

72.2

62.5

74.3

30.8

0

74.3

42

45.8

45

Oligodendrocytes

55.6

51.6

16.2

8.8

0

47.1

21

16.7

20

Pectoral fin

44.4

40

39.5

31.4

0

42.4

31.6

25

45

CNS interneurons

89.5

77.1

75.6

56.4

0

72.7

80

75

80.9

Rohon Beard

38.9

25.7

31.7

5.1

0

22.7

35

8.3

28.6

Brain neurons

55.6

65.7

36.5

25.6

0

56.8

70

50

57.1

Heart

16.6

0

9.8

2.9

0

13.6

0

0

9.5

Muscle

19.4

51.4

39

25.6

10

15.9

55

20.8

33.3

Notocord

44.4

37.1

51.2

17.9

18

27.3

0

25

33.3

Embryos scored

n = 38

n = 35

n = 41

n = 39

n = 40

N = 44

n = 20

n = 24

n = 21

  1. A) 1) psox10-1252, 2) psox10-521, 3) psox10-1252Δ(+159–+468, 4) psox10-100, 5) psox1-01252Δ(+1862–+2220)
  2. B) 1) psox10-1252Δ(+1862–+2220) + BCt, 2) psox10-1252Δ(+1862–+2220) + B, 3) psox10-1252Δ(+1862–+2220) + C, 4) psox10-1252Δ(+1862–+2220) + 2–5
  3. Percentage of embryos positive for GFP in defined region.
  4. Named sox10:GFP constructs were injected into 1-cell stage zebrafish embryos. GFP expression in specific cell-types for each embryo was documented at 48 hpf and 96 hpf. Position and morphology of GFP expressing cells were scored at 600× magnification for individual GFP-expressing live embryos selected at random.
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BMC Developmental Biology

ISSN: 1471-213X

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