Figure 7

Sequence at the 3' end of the zebrafish sox10 intron 1 is essential for sox10 :GFP expression. Cohorts of zebrafish injected with constructs containing sox10 regions at the 3' end of intron 1 as shown were used to identify a minimal sequence essential for sox10:GFP expression. Intron 1 fragments were replaced into an inactive sox10:GFP construct and injected embryos examined for GFP expression as previously described in Fig. 4. The transcription factor binding sites identified by TRANSFAC analysis within this region are illustrated by coloured arrows: red (NFKappaB), yellow (Tcf/Lef), green (FoxD3) and blue (Sox family). The transcription factor binding sites within the sequence that are highly conserved in vertebrate sox10 promoter regions are marked (*). m, muscle n, notochord.