Figure 4

Deletion analysis of the zebrafish sox10 promoter in transient transgenic embryos. A deletion series of sox10:GFP constructs was prepared, with the 5'end of each construct denoted in base pairs 5' of the transcript start (indicated by bent arrows). Please note that -2425sox10:GFP here is equivalent to p4.9:GFP [19]. sox10 exons 1 and 2 are denoted by shaded boxes, with exon 2 fused to GFP (open arrow). Transient transgenic zebrafish were made using each of the sox10:GFP constructs shown and all injected fish were examined and any GFP visible by fluorescent microscopy under a dissecting microscope recorded at 48 hpf. Cohorts of GFP positive embryos were further examined under higher power to determine if the GFP expression corresponded to an expected sox10-like pattern at 48 hpf and again at 96 hpf. Refer to Table 2 for quantitation of GFP expression by cell-type. m muscle, n notochord, s skin.