Figure 1

Genomic context of the zebrafish sox10 5' region used in sox10 :GFP constructs. A. Restriction map constructed following partial sequencing of PAC BUSMP706I16137Q2 (RZPD) showing the location of DNA encoding sox10 (arrow). B. Genomic arrangement of sox10 coding region. The bent arrow shows the transcript start position. Closed boxes illustrate coding sequence and open boxes 5' and 3' untranslated regions. C. 5' upstream genomic region of sox10 used in construction of sox10:GFP plasmids. In B and C a dashed line indicates intron one. Start positions of psox10^-4725:GFP and psox10^-1252:GFP are indicated. B BamHI, E EcoRI, RV EcoRV, N NotI, M MluI, Nh Nhe1, Nc NcoI, Nd NdeI, S SmaI, Sp SpeI, Sh, SphI.