dSTIM has downstream effects on Wg signalling. Immunofluorescent staining of 3rd instar larval wild-type (A-D) wing discs and discs expressing dSTIMFLinduced by sd.Gal4 ((E-H), with antibodies to detect Wg (A, E), Cut (B, F), Ac (C, G) and Dll (D, H). Expression of each protein is reduced at the dorsal/ventral boundary (arrow) except for expression of Wg, Cut and Ac in the central area close to the anterior-posterior boundary (arrowheads). Immunofluorescent staining of basal areas of wing discs expressing dSTIMFL(I-K) and dSTIMRNAi(L-K) induced by MS1096.Gal4 with antibodies to detect dSTIM (I, L) and Arm (J, M). Merged images show dSTIM (green) and Arm (red) (K, N). Expression of Arm is markedly reduced in cells overexpressing dSTIMFL (J, K), but is not affected by expression of dSTIMRNAi(M, N).