DISH for TH components, early neurogenic markers and mature neuronal markers. Cells were labeled in ovo with [3H]-thymidine, and tissue was harvested and dissociated at the indicated times. DISH for TRb, Otx2, Dio3, Crx, or NeuroD and autoradiography for [3H] were carried out on the dissociated cells. (A) The percentage of Otx2+, NeuroD1+, or TRb+ cells that are also [3H] + at increasing times after [3H]-thymidine addition at Stage 26. (B) The percentage of [3H]+ cells, after a 1 hour labeling time, that were also Otx2+, NeuroD1+, TRb+, Dio3+ or Crx+ are shown for Stage 26, Stage 28 and Stage 32. (C) The percentage of Otx2+, NeuroD1+, TRb+, Dio3+ or Crx+ cells that were also [3H]+ after a 1 hour labeling time are shown for Stage 26, Stage 28 and Stage 32.