Figure 8From: Thyroid hormone components are expressed in three sequential waves during development of the chick retinaDISH for TH components, early neurogenic markers and mature neuronal markers. Cells were labeled in ovo with [3H]-thymidine, and tissue was harvested and dissociated at the indicated times. DISH for TRb, Otx2, Dio3, Crx, or NeuroD and autoradiography for [3H] were carried out on the dissociated cells. (A) The percentage of Otx2+, NeuroD1+, or TRb+ cells that are also [3H] + at increasing times after [3H]-thymidine addition at Stage 26. (B) The percentage of [3H]+ cells, after a 1 hour labeling time, that were also Otx2+, NeuroD1+, TRb+, Dio3+ or Crx+ are shown for Stage 26, Stage 28 and Stage 32. (C) The percentage of Otx2+, NeuroD1+, TRb+, Dio3+ or Crx+ cells that were also [3H]+ after a 1 hour labeling time are shown for Stage 26, Stage 28 and Stage 32.Back to article page