Figure 6
From: Inhibition of primordial germ cell proliferation by the medaka male determining gene Dmrt1bY
Shift of male primordial gonad replication pattern towards female by down regulation of Dmrt1bY (BrdU incorporation). Replication pattern of the primordial gonad was followed by BrdU incorporation. A: female primordial gonad phenotype at hatching stage. B: male primordial gonad phenotype at hatching stage. C, D and E: fluorescence produced by BrdU incorporation colocalizes with GFP fluorescence of primordial germ cells in the Olvas transgenic line. GFP fluorescence (C), BrdU fluorescence (D), overlay (E). F: primordial gonad phenotype at hatching stage after Dmrt1bY morpholino injection in a genetic male.