Figure 2
From: Inhibition of primordial germ cell proliferation by the medaka male determining gene Dmrt1bY
Nuclear localization of Dmrt1bY:GFP and ΔDmrt1bY fusion proteins and modulation of cell proliferation by Dmrt1bY overexpression. A and B: When capped RNAs encoding fusion protein of GFP with either Dmrt1bY (A) or ΔDmrt1bY (B) were injected into one cell stage embryos (75 ng/μL) strong nuclear localization is observed (A); mainly cytoplasmic (B) at stage 8. D, E and F: Green fluorescence produced by Dmrt1bY:GFP fusion protein colocalizes with DAPI staining of nuclei. GFP fluorescence (D), DAPI fluorescence (E), overlay (F). C and C': Embryos injected with high doses of Dmrt1bY:GFP, once arrested shortly after MBT, remain quiescent and become necrotic (C'). C: GFP-injected control embryo. G and H: Dmrt1bY injected embryos were stained for the mitotic marker phospho-Histone H3. Wild type embryo (G), Dmrt1bY injected embryo (H). Scale bars: 25 μM in A, B, D, E, F and 120 μM in C, C', G, H.