Figure 2

GATA-2 transgene constructs. (A) A schematic diagram of the zebrafish GATA-2 locus shows the first three exons and approximately 14 kbp of the genomic region upstream of GATA-2. (B) BAC/GFP: GFP modified BAC showing reporter GFP gene inserted before GATA-2 translational start codon. (C) GFP reporter gene construct contains a 7.3 kbp GATA-2 promoter. (D) BACΔUp1/GFP is a GFP modified zebrafish GATA-2 BAC having a deletion of the conserved non-coding sequence Up1. (E) BACΔUp2/GFP is a GFP modified GATA-2 BAC having a deletion of conserved non-coding sequence Up2. (F) BACΔUp1ΔUp2/GFP is a GFP modified GATA-2 BAC having deletions of conserved non-coding sequences Up1 and Up2. (G) mp/GFP is a Tol2 plasmid vector containing GFP linked to a GATA-2 minimal promoter, (H) Up1Up2-mp/GFP is a Tol2 plasmid vector containing Up1 and Up2 linked to mp/GFP, (I) Up1-mp/GFP is a Tol2 plasmid vector containing Up1 linked to mp/GFP, (J) Up2-mp/GFP is a Tol2 plasmid vector containing linked to mp/GFP, (K) Up2a-mp/GFP is a containing Up2a linked to mp/GFP, and (L) Up2b-mp/GFP is a Tol2 plasmid vector containing Up2b linked to mp/GFP. In each Tol2 plasmid vector, GFP reporter gene constructs were inserted between Tol2 flanking sequences. Black block: Up1; Horizontal striped block: Up2; Open block: 5' UTR of zebrafish GATA-2 gene; Diagonal striped block: coding sequence of zebrafish GATA-2 gene.