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Table 5 Quantitative reverse transcription PCR (qRT-PCR) measurements of gene expression in total RNA from longissimus muscle of bovine fetuses – gene expression ratios between breeds

From: Gene expression studies of developing bovine longissimusmuscle from two different beef cattle breeds

Gene

Fold Ratio1

 

P60d/W60d

P135d/W135d

P195d/W195d

Pbirth/Wbirth

Myostatin (GDF8)

1.52 **

1.45 *

1.15

1.51 **

Fatty-acid binding protein 4 (FABP4)

1.32

0.56

0.14 **

0.20 **

Fatty-acid binding protein 5 (FABP5)

0.88

1.09

1.00

0.54 **

Matrin 3 (MATR3)

1.10

1.10

0.96

0.75

  1. 1To obtain fold ratios, mean normalized (from ANOVA and against 18S RNA) qRT-PCR values from P (Piedmontese × Hereford) samples were divided by those from W (Wagyu × Hereford) samples at 60 d gestation, 135 d gestation, 195 d gestation and birth.
  2. * Significance at P < 0.05; ** Significance at P < 0.01.