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Table 5 Quantitative reverse transcription PCR (qRT-PCR) measurements of gene expression in total RNA from longissimus muscle of bovine fetuses – gene expression ratios between breeds

From: Gene expression studies of developing bovine longissimusmuscle from two different beef cattle breeds

Gene Fold Ratio1
  P60d/W60d P135d/W135d P195d/W195d Pbirth/Wbirth
Myostatin (GDF8) 1.52 ** 1.45 * 1.15 1.51 **
Fatty-acid binding protein 4 (FABP4) 1.32 0.56 0.14 ** 0.20 **
Fatty-acid binding protein 5 (FABP5) 0.88 1.09 1.00 0.54 **
Matrin 3 (MATR3) 1.10 1.10 0.96 0.75
  1. 1To obtain fold ratios, mean normalized (from ANOVA and against 18S RNA) qRT-PCR values from P (Piedmontese × Hereford) samples were divided by those from W (Wagyu × Hereford) samples at 60 d gestation, 135 d gestation, 195 d gestation and birth.
  2. * Significance at P < 0.05; ** Significance at P < 0.01.