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Table 4 Quantitative reverse transcription PCR (qRT-PCR) measurements of gene expression in total RNA from longissimus muscle of bovine fetuses – gene expression ratios over developmental time

From: Gene expression studies of developing bovine longissimusmuscle from two different beef cattle breeds

Gene

Fold Ratio1

 

135 d/60 d

195 d/60 d

birth/60 d

IGF-binding protein 5 (IGFBP5)

0.97

0.55 **

0.08 **

Follistatin-like (FSTL1)

0.62 **

0.24 **

0.05 **

  1. 1To obtain fold ratios, mean normalized (from ANOVA and against 18S RNA) qRT-PCR values at 135 d gestation, 195 d gestation and birth were divided by the values at 60 d gestation.
  2. ** Significance at P < 0.01.
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BMC Developmental Biology

ISSN: 1471-213X

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