Gene expression studies of developing bovine longissimusmuscle from two different beef cattle breeds

Table 1 Genes showing decreasing expression in longissimus muscle of bovine fetuses over developmental time¹

Gene²	GenBank Accession³	Number of array elements⁴	Gene expression ratio⁵ 135 d/60 d	Gene expression ratio⁵ 195 d/60 d	Gene expression ratio⁵ birth/60 d
cadherin 11, type 2, OB-cadherin (osteoblast) (CDH11)	DW521798	1	0.26**	0.23**	0.10**
collagen, type I, alpha 1 (COL1A1)	CF613531	14	0.87	0.74	0.16**
collagen, type I, alpha 2 (COL1A2)	CF613950	9	0.75	0.64**	0.15**
collagen, type III, alpha 1 (COL3A1)	CF613798	29	0.72	0.78	0.15**
collagen, type XII, alpha 1 (COL12A1)	CF614200	1	0.48**	0.23**	0.12**
collagen, type XV, alpha 1(COL15A1)	DW521810	1	0.58**	0.41**	0.18**
fibrillin 1 (FBN1)	CF765332	4	0.57**	0.60**	0.23**
fibronectin 1 (FN1)	CF614211	7	0.39**	0.36**	0.08**
follistatin-like 1 (FSTL1)	CF613847	1	0.61**	0.35**	0.13**
guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 (GNAI1)	DW521797	1	0.58**	0.50**	0.20**
glypican 3 (GPC3)	DW521793	1	0.91	0.84	0.11**
Insulin-like growth factor binding protein 5 (IGFBP5)	CF614176	1	0.71	0.72	0.21**
lumican (LUM)	CF613654	2	0.79	0.64**	0.16**
osteoglycin (OGN)	CF614268	1	0.59**	0.67*	0.19**
plastin 3 (T isoform) (PLS3)	DW521782	1	0.49**	0.43**	0.20**
osteonectin (SPARC)	CF613734	8	0.69*	0.68*	0.21**
osteopontin (SPP1)	DW521777	3	0.53**	0.19**	0.15**
stathmin 1/oncoprotein 18 (STMN1)	DW521774	1	0.54**	0.38**	0.10**
tubulin, alpha 1 (TUBA1)	CF613655	2	0.54**	0.46**	0.17**
vimentin (VIM)	CF613639	7	0.51**	0.49**	0.16**

¹* indicates a statistically significant decrease in gene expression at P < 0.05 level; ** indicates a statistically significant decrease in gene expression at P < 0.01 level.
²Sequence annotation was carried out using approved gene symbols (human genome nomenclature) via the IBISS4 database [17]. BLAST [15] scores > 110 and e-values < 2 E-23 were accepted for annotation purposes.
³in cases where more than 1 microarray element was used to calculate the expression data, the accession number of 1 representative element is shown.
⁴Number of microarray elements representing the same gene that were used to calculate the expression data.
⁵To obtain the ratios shown, averaged absolute microarray signal intensity values for d 135, 195 and birth were divided by the signal intensities measured at d 60.

ISSN: 1471-213X