Skip to main content

Table 4 Differentially expressed proteins in the Rana catesbeiana tail fin due to T3-induction as analyzed by iTRAQ

From: Analysis of the Rana catesbeiana tadpole tail fin proteome and phosphoproteome during T3-induced apoptosis: identification of a novel type I keratin

Protein name [accession #/Species]1 Fold change2 Observed peptide mass (Da, iTRAQ [M+H]+)3 Observed peptide sequence4 % Confidence5 Matched database sequence6 E value7
INCREASED
MGC80395 protein (Sterol regulatory element-binding transcription factor 2) [AAH72922/Xenopus laevis] 1.6 2949.6 LTPATVET (frag.) manual Query 1 LTPATVET 8
      LTPATV+T
Sbjct 184 LTPATVQT 191
136
Inter-α inhibitor H4 [XP_848765/Canis familiaris] 2.1 1789.1 V(TFE)LVYEEMLK 90–100 (53)  Query 1 VTFELVYEEMLK 12
      VTFELVYEE+LK
Sbjct 140 VTFELVYEELLK 151
0.004
Hemoglobin α-III chain, larval [P02011/Rana catesbeiana] 1.5 1224.7 FLSFPQTK (frag.) manual Query 1 FLSFPQTK 8
      FLSFPQTK
Sbjct 33 FLSFPQTK 40
5.4
Hemoglobin α-III chain, larval [P02011/Rana catesbeiana] 1.5 1250.8 FLSFPQTK (frag.) 100 Query 1 FLSFPQTK 8
      FLSFPQTK
Sbjct 33 FLSFPQTK 40
5.4
Hemoglobin α-III chain, larval [P02011/Rana catesbeiana] 1.5 2067.0 YVPHFDLTPGSADLNK 99  Query 1 YVPHFDLTPGSADLN 15
      Y PHFDLTPGSADLN
Sbjct 42 YFPHFDLTPGSADLN 56
9 e-05
MGC80107 protein (Biliverdin reductase B) [AAH72790/Xenopus laevis] 1.5 1904.3 VISTPDLSHFFLR 100  Query 1 VISTPDLSHFFLR 13
      VIST DLS FFLR
Sbjct 174 VISTHDLSLFFLR 186
0.26
Immunoglobulin heavy chain [AAC12909/Hydrolagus colliei] 1.6 1237.8 VVLLPPSPK 99 Query 1 VVLLPPSP 8
      V+LLPPSP
Sbjct 133 VILLPPSP 140
63
Immunoglobulin heavy prechain [CAA33212/Xenopus laevis] 2.0 1817.9 SDPDQGFDGTYTVK manual  Query 1 SDPDQGFDGTYTVK 14
      S P++ +DGT+TVK
Sbjct 393 SAPEKAYDGTFTVK 406
52
Immunoglobulin heavy chain constant region [AAC12914/Hydrolagus colliei] 4.6 1391.7 LNVADWNSGK 99  Query 1 LNVA--DWNSGK 10
     LNV+ DW SGK
Sbjct 69 LNVSTEDWKSGK 80
170
Immunoglobulin M heavy chain [AAO37747/Ornithorhynchus anatinus] 1.5 2065.0 FTCTVSHSDLPAPVEK 95  Query 1 FTCTVSHSDLPAP 13
      FTCTVSH+DLPAP
Sbjct 446 FTCTVSHADLPAP 458
7 e-04
Immunoglobulin heavy chain variable region [AAP41191/Lepus granatensis] 1.6 1930.3 (RKQ)VVEAGGALIK 100 (92–96)  Query 3 QVVEEAGGALIK 14
      Q VEE+GG LIK
Sbjct 1 QVEESGGGLIK 12
22
Immunoglobulin heavy prechain [CAA33212/Xenopus laevis] 1.6 1471.8 DQGFDGTYTVK manual  Query 4 FDGTYTVK 11
      +DGT+TVK
Sbjct 399 YDGTFTVK 406
9.2
LOC443721 protein (cathepsin D) [AAH94178/Xenopus laevis] 1.7 980.5 AYWQIR 98 (MALDI) Query 1 AYWQIR 6
      AYWQIR
Sbjct 257 AYWQIR 262
42
DECREASED
α-2-macroglobulin [AAY98517/Xenopus laevis] 0.67 2195.2 AYVTV(LGD)IMGTALENLDR 97–100 (41)    Query 1 AYVTVLGDIMGTALENLDR 19
          AYVTVLGDIMGTA++NLDR
Sbjct 958 AYVTVLGDIMGTAMQNLDR 976
1 E-08
Calcium-binding protein p26olf [BAA34388/Rana catesbeiana] 0.47 1312.7 GNTTSMNFK manual Query 1 GNTTSMNFK 9
      GNTTSMNFK
Sbjct 31 GNTTSMNFK 39
0.57
α1type I collagen [BAA29028/Rana catesbeiana] 0.52 2111.2 TGPAGAPGQDGRPGPPGPPGAR manual  Query 1 TGPAGAPGQDGRPGPPGPPGAR 22
        TGPAGAPGQDGRPGPPGPPGAR
Sbjct 538 TGPAGAPGQDGRPGPPGPPGAR 559
6 e-12
α1type I collagen [BAA29028/Rana catesbeiana] 0.52 2418.2 PPGPSGEK (frag.) 97 Query 1 PPGPSGEK 8
      PPGPSGEK
Sbjct 912 PPGPSGEK 919
17
Caridac α actin 2 [AAX85445/Rana catesbeiana] 0.38 2244.3 VAPEEH(PT)LLTEAPLNPK 93–100 (66)  Query 1 VAPEEHPTLLTEAPLNPK 18
      VAPEEHPTLLTEAPLNPK
 Sbjct 98 VAPEEHPTLLTEAPLNPK 115
3 e-09
myosin heavy chain (skeletal muscle MHC-3) [AAD13771/Rana catesbeiana] 0.39 2491.9 FQAALEEAEASLEHEEAK manual   Query 1 FQAALEEAEASLEHEEAK 18
       FQAALEEAEASLEHEEAK
Sbjct 320 FQAALEEAEASLEHEEAK 337
8 e-09
Mylpf-prov protein (myosin light chain 2) [AAH41503/Xenopus laevis] 0.38 2098.2 NICYVITHGED (frag.) 100  Query 1 NICYVITHGED 11
      NICYVITHGED
Sbjct 156 NICYVITHGED 166
0.002
MGC68533 protein (coatomer protein complex, subunit γ) [AAH61661/Xenopus laevis] 0.61 1491.6 NAHSLYLAGVFR 99 (MALDI)  Query 1 NAHSLYLAGVFR 12
      NAHSLYLAGVFR
Sbjct 824 NAHSLYLAGVFR 835
0.002
Cortactin [NP_005222/Homo sapiens] 0.65 1585.9 YGLFPANYVELR 98 (MALDI)  Query 1 YGLFPANYVELR 12
     YGLFPANYVELR
Sbjct 538 YGLFPANYVELR 549
4 e-04
Triose phosphate isomerase [NP_788764/Drosophila melanogaster] 0.64 1711.8 DIGADWVILGHSER 100 (MALDI)  Query 1 DIGADWVILGHSER 14
      DIGADWVILGHSER
Sbjct 185 DIGADWVILGHSER 198
7 e-06
  1. 1Name of protein for the highest scoring match that resulted with the observed peptide sequence using BLASTp (2.2.15) and the NCBI protein database (Oct. 15, 2006) searching all metazoans. The NCBI accession number and the species name for the identified protein are shown. 2Fold change ratios greater then 1.0 indicate a fold increase, and those below 1.0 indicate the reciprocal of fold decrease. The number is the average fold change of two replicate experiments, which consisted of two treatment samples and two control samples. 3Observed mass of the peptide in the MS analysis, modified with the iTRAQ reagent, reported as singly charged. 4Amino acid sequence determined for the observed peptide by manual de novo or automatic de novo by PEAKS software sequencing, or a MASCOT software match. (frag.) indicates that only a partial peptide sequence could be determined. The masses of isoleucine are indistinguishable from leucine in MS and therefore L can be I and vice versa. 5Percent confidence for the peptide sequence as reported by the PEAKS or MASCOT (MALDI) software. (MALDI) indicates that the peptide was identified in the MALDI-TOF-TOF analysis of iTRAQ samples. "manual" indicates that the peptide sequence was obtained manually and no percent confidence can be reported. 6Indicates the sequence alignment of the observed peptide (Query) to the identified protein (Sbjct) as aligned by BLASTp. The sequence in between indicates the matching and similar (+) amino acids between the two sequences. 7From BLASTp alignments.