Analysis of the Rana catesbeiana tadpole tail fin proteome and phosphoproteome during T3-induced apoptosis: identification of a novel type I keratin

Table 3 Summary of results for iTRAQ analysis by ESI-QqTOF

	Run 1	Run 2	Run 3	Unique¹
Unique spectra ²	7682	6249	6361	n/a
Changing peptides ³	17	17	21	41
*de novo* sequenced ⁴	13	16	18	34
Homology matched ⁵	8	7	11	19
MASCOT ID ⁶	1	1	2	3
Total proteins ID ⁷	11 change:	5 up	6 down

The iTRAQ samples were analyzed three times on an ESI-QqTOF mass spectrometer. ¹Indicates number of unique spectra shared between the replicate runs. ²Total number of unique spectra recorded per MS analysis run. These overlap by unknown amount: n/a. ³Number of spectra that change by ≥ 1.5 fold due to T₃, have good quality reporter tags, and a ratio of the two controls between 0.67 and 1.5. ⁴Number of spectra for which amino acid sequence could be obtained through manual sequencing. ⁵Number of spectra for which a protein inference was made with BLASTp. ⁶Indicates how many spectra were identified using the standard iTRAQ analysis with MASCOT software. ⁷Number of different proteins identified from the resulting peptides and their change in abundance.

ISSN: 1471-213X