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Table 3 Summary of results for iTRAQ analysis by ESI-QqTOF

From: Analysis of the Rana catesbeiana tadpole tail fin proteome and phosphoproteome during T3-induced apoptosis: identification of a novel type I keratin

  Run 1 Run 2 Run 3 Unique1
Unique spectra 2 7682 6249 6361 n/a
Changing peptides 3 17 17 21 41
de novo sequenced 4 13 16 18 34
Homology matched 5 8 7 11 19
MASCOT ID 6 1 1 2 3
Total proteins ID 7 11 change: 5 up 6 down  
  1. The iTRAQ samples were analyzed three times on an ESI-QqTOF mass spectrometer. 1Indicates number of unique spectra shared between the replicate runs. 2Total number of unique spectra recorded per MS analysis run. These overlap by unknown amount: n/a. 3Number of spectra that change by ≥ 1.5 fold due to T3, have good quality reporter tags, and a ratio of the two controls between 0.67 and 1.5. 4Number of spectra for which amino acid sequence could be obtained through manual sequencing. 5Number of spectra for which a protein inference was made with BLASTp. 6Indicates how many spectra were identified using the standard iTRAQ analysis with MASCOT software. 7Number of different proteins identified from the resulting peptides and their change in abundance.