|
Observed peptide mass (Da, [M+H]+)1
|
Peptide sequence from MS/MS2
|
Identified by MALDI-TOF-TOF3
|
% confidence4(MS/MS/MALDI)
|
Matched database sequence5
|
|---|
|
807.4
|
LAADDFR
|
Yes
|
84/89
|
LAADDFR
|
|
809.4
|
LASYLDK
|
Yes
|
100/na
|
LASYLEK
|
|
991.5
|
FENELALR
|
Yes
|
100/98
|
FENELALR
|
|
1041.6
|
LVLQIDNAR
|
Yes
|
100/100
|
VVLQIDNAK
|
|
1073.6
|
ILAATIDNSR
|
Yes
|
100/100
|
ILSATIDNSR
|
|
1079.5
|
VLDELTMSR
|
Yes
|
100/74
|
VLDELTLAR
|
|
1184.6
|
YYDIINDLR
|
-
|
96/-
|
YFEIISDLR
|
|
1202.6
|
QSVEADINGLR
|
-
|
43/-
|
QSVETDINGLR
|
|
1224.6
|
NHEEELQVAR
|
-
|
73/-
|
NHEEEMSIAK
|
|
1232.7
|
-
|
Yes
|
-/100
|
LKFENELALR
|
|
1301.6
|
ALEAANTELELK
|
-
|
93/-
|
ALEAANADLELK
|
- 1Observed peptide masses resulting from the tryptic digestion of the protein spot, reported as singly charged. 2Peptide sequence information deduced from MS/MS spectra of the corresponding peptides from ESI-QqTOF analysis. The masses of isoleucine are indistinguishable from leucine in MS and therefore L can be I and vice versa. 3Indicates which peptides were additionally observed with MALDI-TOF-TOF analysis. 4Percent confidence for the peptide sequences as reported by PEAKS software for the ESI-QqTOF spectra and by MASCOT for MALDI-TOF-TOF data. 5Highest homology match from protein database searching with the observed peptide sequences to X. laevis type I keratin 47 kD using SPIDER software. Bold lettering indicates differences between the observed and database sequences.
