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Table 1 MS analysis of protein spot identified to be a type I keratin fragment

From: Analysis of the Rana catesbeiana tadpole tail fin proteome and phosphoproteome during T3-induced apoptosis: identification of a novel type I keratin

Observed peptide mass (Da, [M+H]+)1 Peptide sequence from MS/MS2 Identified by MALDI-TOF-TOF3 % confidence4(MS/MS/MALDI) Matched database sequence5
807.4 LAADDFR Yes 84/89 LAADDFR
809.4 LASYLDK Yes 100/na LASYLEK
991.5 FENELALR Yes 100/98 FENELALR
1041.6 LVLQIDNAR Yes 100/100 VVLQIDNAK
1073.6 ILAATIDNSR Yes 100/100 ILSATIDNSR
1079.5 VLDELTMSR Yes 100/74 VLDELTLAR
1184.6 YYDIINDLR - 96/- YFEIISDLR
1202.6 QSVEADINGLR - 43/- QSVETDINGLR
1224.6 NHEEELQVAR - 73/- NHEEEMSIAK
1232.7 - Yes -/100 LKFENELALR
1301.6 ALEAANTELELK - 93/- ALEAANADLELK
  1. 1Observed peptide masses resulting from the tryptic digestion of the protein spot, reported as singly charged. 2Peptide sequence information deduced from MS/MS spectra of the corresponding peptides from ESI-QqTOF analysis. The masses of isoleucine are indistinguishable from leucine in MS and therefore L can be I and vice versa. 3Indicates which peptides were additionally observed with MALDI-TOF-TOF analysis. 4Percent confidence for the peptide sequences as reported by PEAKS software for the ESI-QqTOF spectra and by MASCOT for MALDI-TOF-TOF data. 5Highest homology match from protein database searching with the observed peptide sequences to X. laevis type I keratin 47 kD using SPIDER software. Bold lettering indicates differences between the observed and database sequences.