Figure 9

Phosphorylation changes in γ-interferon-inducible lysosomal thiol reductase (GILT). (A) Phosphoprotein 2D gel regions of the mitochondrial and microsomal fractions showing the increase in a row of phosphoprotein spots (s1, s2, s3) due to T₃ treatment at 48 h, while a corresponding total-protein stained 2D region shows no change in the only detectable protein spot s2 (gray arrows). Relative molecular weights of protein standards are indicated in kDa. (B) Spot density measurements (in arbitrary values) are graphed for the corresponding 2D gels on the left. The white bar represents the control while the gray bar represents the T₃ treatment. Error bars represent the standard error of the mean from three independent controls and three independent T₃ samples. Significance is indicated by an asterisk for p < 0.01 and by black dot for p < 0.1 (ANOVA). The values adjacent to the gray bars represent the fold increase due to T₃. Phosphoprotein spots s1, s2, and s3 increase while the corresponding protein spot s2 does not change. MS analysis of the only detectable protein spot s2 (gray arrow) is indicated in table 2. Spot density measurements were normalized between the gels with the β-actin protein spot.