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Figure 8 | BMC Developmental Biology

Figure 8

From: Analysis of the Rana catesbeiana tadpole tail fin proteome and phosphoproteome during T3-induced apoptosis: identification of a novel type I keratin

Figure 8

Changes in transcript and protein fragment levels of RLK I in the tail fin. (A) Fold change in steady-state levels of the keratin transcript relative to time-matched controls after 100 nM T3 exposure for 24, 48 and 72 h. White bars represent controls and gray bars represent T3 treatments. Error bars represent the standard error of the mean (n = 4 for all treatments). Significance is indicated by an asterisk for p < 0.03 (Mann-Whitney U). (B) Fold change in steady-state levels of the keratin transcript at different stages of natural metamorphosis relative to premetamorphic TK stage VI-VIII [31]. Error bars represent the standard error of the mean (n = 4 for all treatments). Significance is indicated by a double asterisk for p < 0.002 (Mann-Whitney U). (C) Immunobloting microsomal fraction samples using anti-pan-cytokeratin antibody reveals the appearance of the keratin fragment (25 kDa) and a concomitant loss of keratin at 50 kDa due to 10 nM T3 treatment. Relative molecular weights of protein standards are indicated in kDa. Shown is a representative of two independent experiments.

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