Expression of 5730521E12Rik in the early developing mouse embryo. 5730521E12Rik expression from E6.5 to E9.5 in the mouse embryo was examined by whole mount in situ hybridization. The embryos at each represented stage are shown in lateral and posterior view (A-D) or lateral and ventral view (E, F), except E9 and E9.5 (G, H). For the lateral view, the embryos are oriented so that the anterior is to left. The expression of 5730521E12Rik is dynamic. Hybridization signals initiate at E7.25 in a few cells in the posterior of the embryos (arrowheads in A), then is continuously detected in broader bilateral domains of the middle-posterior of the embryos at head fold (B) and early somite (C-E) stages. At E8.5–E9 (E-G), 5730521E12Rik expression level reaches the highest in the midgut. At E9.5, the signal is retained but is down-regulated (H).