General profile of bmo-let-7 accumulation during development of silkworm. Both the sense and antisense probes were examined here, but only two antisense probes brought out the same positive results. And lin-4 escaped detection in all samples. All samples were quantified to be 15 μg per well before loading (see Materials and Methods). U6 and 5srRNA hybridizations were used as loading controls and levels of let-7 RNA are quantified relative to 5srRNA. Reproducible and consistent results were confirmed experiments repeated (data not shown).