Figure 5
From: Early specification of dopaminergic phenotype during ES cell differentiation
Quantification of TH expressing neurons derived from monolayer-formed progenitors. Sox1-GFP expressing neural progenitors were sorted from day 7 monolayer cultures and allowed to differentiate for a further 7 days under various conditions. No differences was found in the proportion of TH expressing cells generated by unsorted control cultures, sorted GFPpos neural progenitors re-placed on PA6 or PDL-Laminin coated plastic (A). In unsorted control cultures, the number of TH expressing neurons increased when SHH/FGF8 was applied during differentiation. However, addition of SHH/FGF8 to purified Sox1-GFP cells had no effect on the number of TH expressing neurons produced.