Figure 4

Generation of TH⁺ neurons via monolayer differentiation. A substantial number of neurons start to appear after 10 days of monolayer differentiation (A). After 14 days of differentiation, more mature neurons can be detected (B) and some also express TH expressing (C). Scale bar = 100 μm. Monolayer-derived neural progenitors were identified and isolated based on GFP expression (D-F). TH expressing neurons were generated after a total of 14 days of differentiation when cells were plated on PDL/laminin (G), in co-culture with PA6 cells (H) after sorting as well as in control cultures (I).