Figure 2

Generation of TH⁺ neurons by purified Sox1 -GFP expressing progenitors. Neural progenitors generated by co-culture with PA6 were FACS purified at day 3 or day 7. Following re-plating and differentiation, cultures were fixed and double stained with antibody against β-III-tubulin (green) and TH (red). A similar number of TH expressing neurons was produced by PA6-derived progenitors after being re-plated on PA6 (A) or PDL/Laminin (B) as compared to unsorted controls (C). Sox1-GFP expressing neural progenitors purified at day 3–4 (D, D', D") generated a similar proportion of TH expressing neurons as compared to those isolated at day 7 (E, E', E"). Scale bar = 100 μm.